This cell line is a clonal derivative from the Cas9-Expressing HCT116 Cell Pool (BPS Bioscience, #78073). It was generated by limiting dilution of the original pool and isolation of individual clones, which were screened based on Cas9 expression to obtain a high-expressing (78135-H) or low-expressing (78135-L) cell line. The expressed Cas9 protein includes a C-terminal FLAG tag.

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